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proteinree blocking buffer  (LI-COR)


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    Structured Review

    LI-COR proteinree blocking buffer
    Proteinree Blocking Buffer, supplied by LI-COR, used in various techniques. Bioz Stars score: 98/100, based on 87 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/intercept+protein+free+blocking+buffer/Intercept+(TBS)+Protein-Free+Blocking+Buffer/pm42106918-458-3-6
    Average 98 stars, based on 87 article reviews
    proteinree blocking buffer - by Bioz Stars, 2026-09
    98/100 stars

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    Related Articles

    Membrane:

    Article Title: UBE3A isoform-selective and non-selective contributions to Angelman syndrome phenotypes.
    Article Snippet: Angelman syndrome (AS) is a neurodevelopmental disorder caused by UBE3A loss.. In humans, UBE3A generates three isoforms that localize to distinct subcellular compartments—one mainly nuclear and two cytoplasmic.. The nuclear and most highly expressed cytoplasmic UBE3A isoform are highly conserved in mice, whereas the cytoplasmic human isoform accounting for just ~1% of total UBE3A has no mouse counterpart.

    Article Title: Geranylgeraniol promotes osteoblast differentiation and inhibits osteoclastogenesis through MAPK and nuclear receptor signaling.
    Article Snippet: Samples were separated using SDS-PAGE and transferred onto Immobilon-P PVDF membranes (Millipore, Billerica, MA, USA) for western blot analysis. .. The membrane was blocked with 5% skim milk in TBST (137mM NaCl, 2.68 mM KCl, 25 mM Tris pH 7.4 0.1% Tween 20), or intercept-protein-free blocking buffer (LICORbio), incubated with anti-mouse antibodies for 2‒12 h, and then incubated with horseradishperoxidase-conjugated anti-rabbit antibody. .. Protein bands were detected using Clarity Western ECL Substrate (Bio-Rad, Hercules, CA, USA) or ImmunoStar LD (Fujifilm Wako Pure Chemical), and analyzed using a ChemiDoc MP imaging system (Bio-Rad) or C-DiGit Blot Scanner (LICORbio).

    Blocking Assay:

    Article Title: UBE3A isoform-selective and non-selective contributions to Angelman syndrome phenotypes.
    Article Snippet: Angelman syndrome (AS) is a neurodevelopmental disorder caused by UBE3A loss.. In humans, UBE3A generates three isoforms that localize to distinct subcellular compartments—one mainly nuclear and two cytoplasmic.. The nuclear and most highly expressed cytoplasmic UBE3A isoform are highly conserved in mice, whereas the cytoplasmic human isoform accounting for just ~1% of total UBE3A has no mouse counterpart.

    Article Title: GlycoRNA complexed with heparan sulfate regulates VEGF-A signalling.
    Article Snippet: Post-transfer, the membrane was rinsed in 1× PBS and dried on Whatman Paper (GE Healthcare). .. Dried membranes were rehydrated in Intercept Protein-Free Blocking buffer (LI-COR Biosciences) for 30 min at room temperature. ..

    Article Title: Discovery of a potent anti-Zika virus benzamide series targeting the viral protein NS4B.
    Article Snippet: .. After blocking for one hour with Intercept Protein-Free blocking buffer (Li-Cor), cells were incubated with MWAC-4163 in PBS with 10% blocking buffer for 30 minutes. .. After extensive washing, cells were analyzed on an Accuri C6 flow cytometer (BD Sciences) using channel 1 for FAM (ex488 nm/em533) and channel 4 (ex640 nm/em675LP) for miniRFP.

    Article Title: Discovery of a potent anti-Zika virus benzamide series targeting the viral protein NS4B
    Article Snippet: .. After blocking for one hour with Intercept Protein-Free blocking buffer (Li-Cor), cells were incubated with MWAC-4163 in PBS with 10% blocking buffer for 30 minutes. .. After extensive washing, cells were analyzed on an Accuri C6 flow cytometer (BD Sciences) using channel 1 for FAM (ex488 nm/em533) and channel 4 (ex640 nm/em675LP) for miniRFP.

    Article Title: POU1F1 induces cancer stem cell-like traits in breast cancer cells by IL-6/JAK2/STAT3 activation and enrichment of ALDH.
    Article Snippet: Cell extracts (30 g) were denatured at 95 °C, subjected to SDS-PAGE, and transferred to PVDF membranes (Merck Millipore, Burlington, USA). .. After blocking with Intercept Protein-Free Blocking buffer (LI-COR Biotechnology, Lincoln, USA), membranes were incubated overnight at 4 AR TI CL E IN P RE SS °C with anti-POU1F1 antibody (Sigma), anti-GPDH, and anti--actin (Santacruz), anti- JAK2, anti-pJAK2, anti-STAT3, anti-pSTAT3, anti-ERK1/2, anti-pERK1/2, anti-AKT, and anti-pAKT (Cell Signalling, Danvers, USA,) antibodies for their expression and phosphorylation. .. A fluorescent secondary antibody was then applied, and detection was performed using the Li-Cor Odyssey fluorescence system at 700 and 800 nm wavelengths.

    Article Title: The Role of the Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) in Enamel Formation.
    Article Snippet: Samples were loaded onto 4%–20% Mini-PROTEAN TGX SDS-PAGE gels (Bio-Rad, 4561094) and subjected to electrophoresis with Tris/glycine/SDS running buffer at 100 V for 90 min. Gels were then either stained with Coomassie Brilliant Blue or transferred to PVDF membranes (InvitrogenTM IB401001) using an Invitrogen iBlotTM Gel Transfer Device. .. Membranes were blocked with Intercept Protein Free Blocking Buffer (LI-COR, 927-90001) and then incubated overnight with either anti-AMELX or anti-KLK4 antibodies (see Supplemental Table 2 for dilutions). ..

    Article Title: Geranylgeraniol promotes osteoblast differentiation and inhibits osteoclastogenesis through MAPK and nuclear receptor signaling.
    Article Snippet: Samples were separated using SDS-PAGE and transferred onto Immobilon-P PVDF membranes (Millipore, Billerica, MA, USA) for western blot analysis. .. The membrane was blocked with 5% skim milk in TBST (137mM NaCl, 2.68 mM KCl, 25 mM Tris pH 7.4 0.1% Tween 20), or intercept-protein-free blocking buffer (LICORbio), incubated with anti-mouse antibodies for 2‒12 h, and then incubated with horseradishperoxidase-conjugated anti-rabbit antibody. .. Protein bands were detected using Clarity Western ECL Substrate (Bio-Rad, Hercules, CA, USA) or ImmunoStar LD (Fujifilm Wako Pure Chemical), and analyzed using a ChemiDoc MP imaging system (Bio-Rad) or C-DiGit Blot Scanner (LICORbio).

    Article Title: GlycoRNA complexed with heparan sulfate regulates VEGF-A signalling
    Article Snippet: Post-transfer, the membrane was rinsed in 1× PBS and dried on Whatman Paper (GE Healthcare). .. Dried membranes were rehydrated in Intercept Protein-Free Blocking buffer (LI-COR Biosciences) for 30 min at room temperature. ..

    Incubation:

    Article Title: UBE3A isoform-selective and non-selective contributions to Angelman syndrome phenotypes.
    Article Snippet: Angelman syndrome (AS) is a neurodevelopmental disorder caused by UBE3A loss.. In humans, UBE3A generates three isoforms that localize to distinct subcellular compartments—one mainly nuclear and two cytoplasmic.. The nuclear and most highly expressed cytoplasmic UBE3A isoform are highly conserved in mice, whereas the cytoplasmic human isoform accounting for just ~1% of total UBE3A has no mouse counterpart.

    Article Title: Discovery of a potent anti-Zika virus benzamide series targeting the viral protein NS4B.
    Article Snippet: .. After blocking for one hour with Intercept Protein-Free blocking buffer (Li-Cor), cells were incubated with MWAC-4163 in PBS with 10% blocking buffer for 30 minutes. .. After extensive washing, cells were analyzed on an Accuri C6 flow cytometer (BD Sciences) using channel 1 for FAM (ex488 nm/em533) and channel 4 (ex640 nm/em675LP) for miniRFP.

    Article Title: Discovery of a potent anti-Zika virus benzamide series targeting the viral protein NS4B
    Article Snippet: .. After blocking for one hour with Intercept Protein-Free blocking buffer (Li-Cor), cells were incubated with MWAC-4163 in PBS with 10% blocking buffer for 30 minutes. .. After extensive washing, cells were analyzed on an Accuri C6 flow cytometer (BD Sciences) using channel 1 for FAM (ex488 nm/em533) and channel 4 (ex640 nm/em675LP) for miniRFP.

    Article Title: POU1F1 induces cancer stem cell-like traits in breast cancer cells by IL-6/JAK2/STAT3 activation and enrichment of ALDH.
    Article Snippet: Cell extracts (30 g) were denatured at 95 °C, subjected to SDS-PAGE, and transferred to PVDF membranes (Merck Millipore, Burlington, USA). .. After blocking with Intercept Protein-Free Blocking buffer (LI-COR Biotechnology, Lincoln, USA), membranes were incubated overnight at 4 AR TI CL E IN P RE SS °C with anti-POU1F1 antibody (Sigma), anti-GPDH, and anti--actin (Santacruz), anti- JAK2, anti-pJAK2, anti-STAT3, anti-pSTAT3, anti-ERK1/2, anti-pERK1/2, anti-AKT, and anti-pAKT (Cell Signalling, Danvers, USA,) antibodies for their expression and phosphorylation. .. A fluorescent secondary antibody was then applied, and detection was performed using the Li-Cor Odyssey fluorescence system at 700 and 800 nm wavelengths.

    Article Title: The Role of the Cystic Fibrosis Transmembrane Conductance Regulator (CFTR) in Enamel Formation.
    Article Snippet: Samples were loaded onto 4%–20% Mini-PROTEAN TGX SDS-PAGE gels (Bio-Rad, 4561094) and subjected to electrophoresis with Tris/glycine/SDS running buffer at 100 V for 90 min. Gels were then either stained with Coomassie Brilliant Blue or transferred to PVDF membranes (InvitrogenTM IB401001) using an Invitrogen iBlotTM Gel Transfer Device. .. Membranes were blocked with Intercept Protein Free Blocking Buffer (LI-COR, 927-90001) and then incubated overnight with either anti-AMELX or anti-KLK4 antibodies (see Supplemental Table 2 for dilutions). ..

    Article Title: Geranylgeraniol promotes osteoblast differentiation and inhibits osteoclastogenesis through MAPK and nuclear receptor signaling.
    Article Snippet: Samples were separated using SDS-PAGE and transferred onto Immobilon-P PVDF membranes (Millipore, Billerica, MA, USA) for western blot analysis. .. The membrane was blocked with 5% skim milk in TBST (137mM NaCl, 2.68 mM KCl, 25 mM Tris pH 7.4 0.1% Tween 20), or intercept-protein-free blocking buffer (LICORbio), incubated with anti-mouse antibodies for 2‒12 h, and then incubated with horseradishperoxidase-conjugated anti-rabbit antibody. .. Protein bands were detected using Clarity Western ECL Substrate (Bio-Rad, Hercules, CA, USA) or ImmunoStar LD (Fujifilm Wako Pure Chemical), and analyzed using a ChemiDoc MP imaging system (Bio-Rad) or C-DiGit Blot Scanner (LICORbio).

    Saline:

    Article Title: UBE3A isoform-selective and non-selective contributions to Angelman syndrome phenotypes.
    Article Snippet: Angelman syndrome (AS) is a neurodevelopmental disorder caused by UBE3A loss.. In humans, UBE3A generates three isoforms that localize to distinct subcellular compartments—one mainly nuclear and two cytoplasmic.. The nuclear and most highly expressed cytoplasmic UBE3A isoform are highly conserved in mice, whereas the cytoplasmic human isoform accounting for just ~1% of total UBE3A has no mouse counterpart.

    Expressing:

    Article Title: POU1F1 induces cancer stem cell-like traits in breast cancer cells by IL-6/JAK2/STAT3 activation and enrichment of ALDH.
    Article Snippet: Cell extracts (30 g) were denatured at 95 °C, subjected to SDS-PAGE, and transferred to PVDF membranes (Merck Millipore, Burlington, USA). .. After blocking with Intercept Protein-Free Blocking buffer (LI-COR Biotechnology, Lincoln, USA), membranes were incubated overnight at 4 AR TI CL E IN P RE SS °C with anti-POU1F1 antibody (Sigma), anti-GPDH, and anti--actin (Santacruz), anti- JAK2, anti-pJAK2, anti-STAT3, anti-pSTAT3, anti-ERK1/2, anti-pERK1/2, anti-AKT, and anti-pAKT (Cell Signalling, Danvers, USA,) antibodies for their expression and phosphorylation. .. A fluorescent secondary antibody was then applied, and detection was performed using the Li-Cor Odyssey fluorescence system at 700 and 800 nm wavelengths.

    Phospho-proteomics:

    Article Title: POU1F1 induces cancer stem cell-like traits in breast cancer cells by IL-6/JAK2/STAT3 activation and enrichment of ALDH.
    Article Snippet: Cell extracts (30 g) were denatured at 95 °C, subjected to SDS-PAGE, and transferred to PVDF membranes (Merck Millipore, Burlington, USA). .. After blocking with Intercept Protein-Free Blocking buffer (LI-COR Biotechnology, Lincoln, USA), membranes were incubated overnight at 4 AR TI CL E IN P RE SS °C with anti-POU1F1 antibody (Sigma), anti-GPDH, and anti--actin (Santacruz), anti- JAK2, anti-pJAK2, anti-STAT3, anti-pSTAT3, anti-ERK1/2, anti-pERK1/2, anti-AKT, and anti-pAKT (Cell Signalling, Danvers, USA,) antibodies for their expression and phosphorylation. .. A fluorescent secondary antibody was then applied, and detection was performed using the Li-Cor Odyssey fluorescence system at 700 and 800 nm wavelengths.



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